| 糖化酶酶解米渣纯化米蛋白的工艺 |
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| 引用本文： | 舒德海,钱俊青. 糖化酶酶解米渣纯化米蛋白的工艺[J]. 氨基酸和生物资源, 2009, 31(3): 7-9,14 |
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| 作者姓名： | 舒德海 钱俊青 |
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| 作者单位： | 浙江工业大学药学院,杭州,浙江,310014;浙江工业大学药学院,杭州,浙江,310014 |
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| 摘 要： | 研究了糖化酶酶解米渣纯化米蛋白的实验条件:液固比、酶解时间、pH、温度和酶量。通过正交实验优化了酶解主要条件,得到糖化酶水解米渣最佳条件:液固比5:1,时间3 h,pH 4.0,温度65℃和酶量30 U.g-1。在最佳条件下实验,米蛋白的提取率为81.3%,纯度为76.8%。
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| 关 键 词： | 米蛋白 酶解 糖化酶 纯化 |
Technology of Purifying Rice Protein from Rice Residue through the Enzymolysis of Diastase |
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| QIAN Jun-qing,SHU De-hai. Technology of Purifying Rice Protein from Rice Residue through the Enzymolysis of Diastase[J]. Amino Acids & Biotic Resources, 2009, 31(3): 7-9,14 |
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| Authors: | QIAN Jun-qing SHU De-hai |
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| Affiliation: | QIAN Jun-qing,SHU De-hai(College of Pharmaceutical,Zhejiang University of Technology,Hangzhou 310014,China) |
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| Abstract: | Purifying rice protein from rice residue by diastase was studied,and ratio of liquid and solid(V:W),enzymolysis time,pH value,temperature and enzyme quantity were tested.Through orthogonal experiment,the conditions of enzymolysis were optimized:V:W=5,3hours,pH4.0,65℃ and 30U·g-1.Under the optimum conditions,the extraction yield and purity of rice protein were 81.3% and 76.8%. |
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| Keywords: | rice protein enzymolysis diastase purification |
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