Inhibition of surfactant release from isolated type II cells by compound 4880 |
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Authors: | Ward R. Rice Jeffrey A. Whitsett |
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Affiliation: | University of Cicinnati Medical Center, Pediatrics—Newborn Division, 231 Bethesda Avenue, Cincinnati, OH 45267-0541 U.S.A. |
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Abstract: | Release of [3H]phosphatidylcholine from pulmonary Type II epithelial cells was stimulated by terbutaline, forskolin and cytochalasin D. Compound inhibited both basal and agonist-stimulated release of [3H]PC. The IC50 for inhibition by compound was 1–2 μg/ml, and was similar for inhibition of both basal and stimulated release of [3H]phosphatidylcholine. Inhibitory effects of were noted following a 1 h exposure to compound and persisted up to 3 h. The inhibitory effect of compound was entirely reversed by removing compound from the external milieu. Compound had no effect on cytosolic cyclic AMP levels or lactate dehydrogenase release. Inhibition of surfactant release produced by compound was unaffected by changes in extracellular calcium concentrations. Compound is a non-toxic inhibitor of phosphatidylcholine release from Type II epithelial cells. |
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Keywords: | Surfactant release (Type II cell) PC phosphatidylcholine concentration producing half the maximal inhibition DMSO dimethylsulfoxide EGTA Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid |
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