Ultraviolet-crosslinking reveals specific affinity of eukaryotic initiation factors for Semliki Forest virus mRNA |
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| Authors: | Buddy Setyono Harry Van Steeg Harry O Voorma |
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| Institution: | 1. Biologisches Institut, Universität Stuttgart, Ulmer Strasse 227, D-7000 Stuttgart 60 F.R.G.;2. Vakgroep Moleculaire Celbiologie, Rijksuniversiteit Utrecht, NL-3584-CH Utrecht The Netherlands |
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| Abstract: | Eukaryotic initiation factors (eIF) associate readily with 32P-labeled Semliki Forest virus (SFV) mRNA in vitro, forming complexes which can be crosslinked by 254 nm ultraviolet irradiation. After ribonuclease digestion, the initiation factors were released and analysed by gel electrophoresis. Autoradiography revealed proteins by virtue of crosslinked 32P-labeled mRNA fragments. eIF-4A, -4B and -4C as well as three subunits of eIF-3 could be crosslinked with SFV mRNA. None of these proteins bound to ribosomal RNAs. |
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| Keywords: | Protein synthesis Initiation factor Ultraviolet crosslinking mRNA (Semliki Forest virus) SFV Semliki Forest virus eIF eukaryotic initiation factor DMSO dimethyl sulfoxide |
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