Recombined molecules of mitochondrial DNA obtained from crosses between cytoplasmic petite mutants of Saccharomyces cerevisiae: The stoichiometry of parental DNA repeats within the recombined molecule |
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Authors: | Martine Defais Perrine Caillet-Fauquet Maurice S Fox and Miroslav Radman |
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Institution: | (1) Départment de Biologie Moléculaire, Université Libre de Bruxelles, B 1640 Rhode St Genèse, (Belgium);(2) Biology Department, Massachusetts Institute of Technology, 02139 Cambridge, Mass, (U.S.A.) |
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Abstract: | Summary Ultraviolet mutagenesis of phage is produced by host functions which are inducible by ultraviolet irradiation of the host cell. Induction kinetics and the half life of the inducible mutagenic DNA repair (SOS-repair) in E. coli have been determined using phage assays. At 37°C, both mutagenic and repair activities are maximal approximately 30 min following irradiation and decay with a half life of approximately 30 min. The presence of 100 g/ml chloramphenicol during the first 40 min after irradiation completely abolishes induction of repair and mutagenesis. The ultraviolet induction pattern of SOS repair very much resembles that of prophage in lysogenic induction (Monk and Kinross, 1975). |
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