Detection and analysis of extra- and intracellular deoxyribonuclease activities in Lactobacillus plantarum |
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| Authors: | J.L. Caso,& J.E. Suá rez |
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| Affiliation: | Area de Microbiología, Departamento de Biología Funcional, Universidad de Oviedo, and Instituto Universitario de Biotecnología de Asturias (IUBA), Oviedo, Spain |
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| Abstract: | Extracellular endodeoxyribonuclease activities have been detected in culture supernatant fluids of several Lactobacillus plantarum strains. In the case of Lact. plantarum HER 1325 at least, the extracellular activity is accompanied by a cytoplasmic restriction endonuclease. Among the secreted nucleases, the greatest activity was from Lact. plantarum ATCC 10241. This strain secretes an enzyme, with a molecular mass in the range 10–40 kDa, that cuts double-stranded DNA in a sequence-independent way by initially introducing single-strand nicks in supercoiled molecules, followed by linearization and complete degradation of the substrate. |
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