Monitoring the active transport of efflux pumps after their reconstitution into proteoliposomes: caveats and keys |
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| Authors: | Picard M Verchère A Broutin I |
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| Affiliation: | Laboratoire de Cristallographie et RMN Biologiques (CNRS UMR 8015), Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité 75006 Paris, France. martin.picard@parisdescartes.fr |
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| Abstract: | There is an acute need for a functional assay allowing the investigation of efflux pumps. A dedicated procedure was previously developed, but although it was unambiguous, it suffered from a lack of reproducibility. We describe an optimization of the procedure that makes the assay much more robust. |
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