| 小麦新品种“川麦42”低分子量谷蛋白亚基新基因的分子克隆 |
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| 引用本文： | 沈蕾,龙海,颜泽洪,魏育明,郑有良. 小麦新品种“川麦42”低分子量谷蛋白亚基新基因的分子克隆[J]. 遗传, 2006, 28(1): 57-64 |
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| 作者姓名： | 沈蕾 龙海 颜泽洪 魏育明 郑有良 |
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| 作者单位： | （1.四川农业大学小麦研究所， 都江堰 611830; 2.四川农业大学西南作物基因资源与遗传改良教育部重点实验室，雅安 625014） |
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| 基金项目： | 国家高技术研究发展计划(863计划)，高等学校全国优秀博士学位论文作者专项基金，教育部长江学者和创新团队发展计划 |
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| 摘 要： | 采用PCR方法从小麦（Triticum aestivum L.）新品种“川麦42”中克隆得到一个低分子量谷蛋白亚基（LMW-GS）新基因，暂命名为LMWCM42-1。该基因编码区全长846 bp，编码281个氨基酸，具有LMW-GS基因的典型结构特征。推导氨基酸序列比较显示，尽管LMWCM42-1与已知LMW-GS高度相似，但在N-末端重复区部分重复单元和C-末端区中仍存在明显差异。聚类分析表明，LMWCM42-1可能是由Glu-D3位点编码的。
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| 关 键 词： | 序列分析 低分子量谷蛋白亚基基因 小麦 |
| 文章编号： | 0253-9772(2006)01-0057-08 |
| 收稿时间： | 2004-11-16 |
| 修稿时间： | 2005-03-01 |
Molecular Cloning of A Novel Low-molecular-weight Glutenin Subunit Gene from Wheat Variety "Chuanmai 42" |
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| SHEN Lei,LONG Hai,YAN Ze-Hong,WEI Yu-Ming,ZHENG You-Liang. Molecular Cloning of A Novel Low-molecular-weight Glutenin Subunit Gene from Wheat Variety "Chuanmai 42"[J]. Hereditas, 2006, 28(1): 57-64 |
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| Authors: | SHEN Lei LONG Hai YAN Ze-Hong WEI Yu-Ming ZHENG You-Liang |
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| Affiliation: | (1．Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China； |
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| Abstract: | Using PCR-amplification method,the coding sequences of a novel low-molecular-weight glutenin(LMW-GS) gene,LMWCM42-1,was isolated from the genomic DNA of wheat(Triticum aestivum L.) variety 'Chuanmai 42'.The full coding region of this gene consisted of 846 nucleotides,and encoded a protein with 281 amino acids.LMWCM42-1 is a typical low-molecular-weight glutenin subunit(LMW-GS) gene.In spite of high similarity with the known LMW-GSs,LMWCM42-1 was quite different from the known LMW-GS in the N-terminal,central repetitive domain and C-terminal domain.It was found that LMWCM42-1 was located within the Glu-D3 locus by phylogenetic analysis. |
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| Keywords: | wheat Iow-molecular-werght glutenin subunit gene sequence analysis |
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