Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal |
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| Authors: | Ayyadevara Srinivas Engle Mark R Singh Sharda P Dandapat Abhijit Lichti Cheryl F Benes Helen Shmookler Reis Robert J Liebau Eva Zimniak Piotr |
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| Institution: | Department of Pharmacology & Toxicology, University of Arkansas for Medical Sciences, and Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA. |
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| Abstract: | Caenorhabditis elegans expresses a glutathione transferase (GST) belonging to the Pi class, for which we propose the name CeGSTP2-2. CeGSTP2-2 (the product of the gst-10 gene) has the ability to conjugate the lipid peroxidation product 4-hydroxynonenal (4-HNE). Transgenic C. elegans strains were generated in which the 5'-flanking region and promoter of gst-10 were placed upstream of gst-10 and mGsta4 cDNAs, respectively. mGsta4 encodes the murine mGSTA4-4, an enzyme with particularly high catalytic efficiency for 4-HNE. The localization of both transgenes was similar to that of native CeGSTP2-2. The 4-HNE-conjugating activity in worm lysates increased in the order: control
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| Keywords: | 4-hydroxynonenal aging C elegans longevity regulation molecular biology of aging oxidative stress stress resistance transgenes |
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