Isolation of a DNA polymerase fraction active with single-strand DNA and stimulated by ATP |
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Authors: | E Milewski M Kohiyama |
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Affiliation: | Université Paris VII Institut de Biologio Molécularie Tour 43.2, Place Jussieu 75005 Paris, France |
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Abstract: | A DNA polymerase fraction was isolated from a polA?1 strain. Several characteristics distinguish this activity. The polymerase preparation preferentially uses single-strand DNA including φ× DNA as template. ATP stimulates the rate of synthesis approximately three times. The effect of ATP is specific; other ribonucleotide triphosphates are not effective. The complete temperature sensitivity of the fraction obtained from a dnaE mutant indicates that DNA polymerase III participates in the reaction. |
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