Isolation of a DNA polymerase fraction active with single-strand DNA and stimulated by ATP

A DNA polymerase fraction was isolated from a polA^?₁ strain. Several characteristics distinguish this activity. The polymerase preparation preferentially uses single-strand DNA including φ× DNA as template. ATP stimulates the rate of synthesis approximately three times. The effect of ATP is specific; other ribonucleotide triphosphates are not effective. The complete temperature sensitivity of the fraction obtained from a dnaE mutant indicates that DNA polymerase III participates in the reaction.