Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids |
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Authors: | Barbara Schiller R G Herrmann and G Melchers |
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Institution: | (1) Botanisches Institut der Universität, D-4000 Düsseldorf 1, Federal Republic of Germany;(2) Max-Planck-Institut für Biologie, D-7400 Tübingen, Federal Republic of Germany |
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Abstract: | Summary The buoyant density and endonuclease restriction patterns of potato (Solanum tuberosum L.) and tomato (Lycopersicon esculentum) ptDNA were examined and compared with those of their somatic hybrids. The plastids from these plants, both of which belong to the family of Solanaceae, contain a single DNA species whose density of 1.697 gcm-3 and size of approximately 156 kbp are similar to those of ptDNA from other higher plants. The Sal I restriction patterns were indistinguishable; however, those obtained with Kpn I, Pst I, and Eco RI disclosed that each species possesses a unique ptDNA. These observations suggest a relatively recent divergence of both species. Of the twelve hybrid lines screened, eight contained exclusively potato ptDNa and four contained only tomato ptDNA at a 0.1–3% level of detection. Rearrangements of modifications of DNA were not detected. The plastid identities of three hybrid lines that had previously been analyzed by isoelectric focusing of RuBPcase subunits (Melchers et al. 1978) agreed with those determined by restriction endonuclease analysis.Abbreviations used in the text ptDNA
plastid DNA, chloroplast DNA
- cDNA
copy DNA
- RuBPcase
ribulose bisphosphate carboxylase/oxygenase
- LSU
large subunit of RuBPcase
- kbp
kilobase pairs
- SDS
sodium dodecyl sulfate
- SSC
standard saline citrate
- IEF
isoelectric focusing |
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