Incorporation of 4-14C-22,23-3 H-sitosterol Into diosgenin by Dioscorea deltoidea tissue suspension cultures |
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Authors: | S.J. Stohs J.J. Sabatka H. Rosenberg |
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Affiliation: | Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Nebraska, Lincoln, NB 68508, U.S.A. |
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Abstract: | Sitosterol-4-14C-22,23-3H with a 3H/14C ratio of 5.0 was incorporated into diosgenin such that the 3H/14C ratio in diosgenin was approx. 2.3. The per cent of 14C incorporation was 0· and for 3H was 0·42%. The results indicate that C-23 is not involved in the transformation of sitosterol into diosgenin. The first step in the cyclization of the sterol side-chain may either involve oxygenation at C-26 or direct hydroxylation at C-22 via a mixed function oxidase system. Other indirect evidence suggests that the C-26 oxygenation mechanism is operative. |
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Keywords: | Dioscoreaceae suspension cultures diosgenin biosynthesis sitosterol C-26 oxygenation. |
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