Ferredoxin activation by rhodanese |
| |
| Authors: | Umberto Tomati Rosamarina Matarese Giorgio Federici |
| |
| Affiliation: | Laboratory of Radiobiochemistry and plant Ecophysiology, National Research Council, and the Institute of Biological Chemistry, University of Rome, Rome, Italy |
| |
| Abstract: | Rhodanese was extracted from Brassica oleracea leaves and purified 150-fold. The enzyme was shown to have optimum activity at pH 8-8.5 and a temperature range of 50-55°; a Km of 0.4 mM at 30° for thiosulphate and cyanide. and mol. wt around 32000. The electrophoretically pure enzyme is able to produce the biological and spectral properties of ferredoxin when added to apoferredoxin in the presence of thiosulphate. |
| |
| Keywords: | rhodanese apoferredoxin ferredoxin activation. |
| 本文献已被 ScienceDirect 等数据库收录！ |
