Biosynthesis of gibberellins A12, A15, A24, A36, and A37 by a cell-free system from Cucurbita maxima |
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Authors: | Jan E Graebe Peter Hedden Paul Gaskin Jake MacMillan |
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Institution: | Pflanzenphysiologisches Institut der Universität, D-34 Göttingen, Germany;Department of Organic Chemistry, The University, Bristol, England |
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Abstract: | GA12-aldehyde obtained from mevalonate via ent-kaurene, ent-kaurenol, ent-kaurenoic acid and ent-7α-hydroxykaurenoic acid in a cell-free system from immature seeds of Cucurbita maxima was converted to GA12 by the same system. When Mn2+ was omitted from the system GA12-aldehyde and GA12 were converted further to several products. Among these GA15, GA24, GA36 and GA37 were conclusively identified by GC-MS. With the exception of GA37 these GAs have not previously been found in higher plants. Another biosynthetic pathway led from ent-7α-hydroxykaurenoic acid to very polar products via what was tentatively identified as ent-6α, 7α-dihydroxykaurenoic acid. An unidentified component with an MS resembling that of a dihydroxykaurenolide was also obtained from incubations with mevalonate. |
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Keywords: | Cucurbitaceae biosynthesis cell-free system gibberellins diterpenoids |
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