Detailed analysis of metabolites from mevalonic lactone in Gibberella fujikuroi |
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Authors: | Jake MacMillan Colin M. Wels |
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Affiliation: | School of Chemistry, The University of Bristol, Bristol BS8 ITS England |
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Abstract: | Methods for the detailed analysis of diterpenoid metabolites from Gibberella fujikuroi are described. The metabolites from 2-[3H]-mevalonic acid lactone are separated by partition chromatography on Sephadex LH20 columns, detected by radio-counting and characterised by gas-chromatography directly linked to radio-counting and MS. From one fermentation, 72 compounds were detected. Of these, 25 known diterpenes, including 15 gibberellins, were identified and 7 new products were assigned tentative structures. |
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Keywords: | fungus diterpenoid metabolites gibberellins. |
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