Catechol O-methyltransferases in pampas grass: differentiation of m- and p-methylating activities |
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Authors: | Bernard J. Finkle Stanley H. Kelly |
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Affiliation: | Western Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Berkeley, CA 94710, U.S.A. |
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Abstract: | Partially purified catechol O-methyltransferase from pampas grass (Cortaderia selloana) catalyses the methylations of substrates at both their meta and para positions. This capability was shown, by heat treatments, to arise from a less stable m-O-methyl-transferring activity and a more stable p-O-methyltransferring activity, tested against protocatechuic acid. When acting upon caffeic acid, the preparation catalyses a reaction of solely m-O-methyltransfer (in contrast to the mixed methylation of this substrate exhibited by rat liver catechol O-methyltransferase). A small degree of m-O-methylation of monophenolic substrates also occurs. |
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Keywords: | Gramineae pampas grass hydroxybenzoic acids. |
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