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New plant binary vectors with selectable markers located proximal to the left T-DNA border
Authors:Detlef Becker  Elke Kemper  Jeff Schell  Robert Masterson
Affiliation:(1) Max-Planck-Institut für Züchtungsforschung, Carl von Linné Weg 10, D-5000 Köln 30, Germany
Abstract:Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and beta-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.
Keywords:Agrobacterium tumefaciens  T-DNA  plant transformation  binary vectors  selectable markers
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