Molecular cloning of thentrA gene of the broad host-rangeRhizobium sp. NGR234, and phenotypes of a site-directed mutant |
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| Authors: | John Stanley Jan van Slooten David N. Dowling Turlough Finan William J. Broughton |
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| Affiliation: | (1) Laboratoire de Biologie Moléculaire des Plantes Supérieures, University of Geneva, 1 chemin de l’Impératrice, CH-1292 Chambésy-Genève, Switzerland;(2) Department of Biology, McMaster University, L8S 4K1 Hamilton, Ontario, Canada |
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| Abstract: | Summary The clonedntrA (rpoN) gene andntrA mutants ofRhizobium meliloti were used to isolate the homologous gene from the broad-host rangeRhizobium sp. NGR234 by hybridization and interspecies complementation. The NGR234 locus was analyzed by deletion and insertional mutagenesis. A site-directedntrA mutant, NGR234rn1, was made with an interposon, GmI, and its phenotype was examined ex planta and in symbiosis. NGR234rn1 formed Fix− nodules on six genera tested from among its legume hosts, including both indeterminate and determinate nodule-type plants. Formation of nodules onMacroptilium was delayed, and expression of anR. meliloti nodABC-lacZ fusion was reduced by the mutant allele. |
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| Keywords: | Sigma factor Interposon Symbiosis lacZ-fusions |
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