| 东方田鼠肝脏cDNA 文库的构建及部分克隆序列分析 |
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| 引用本文： | 冯洁,谢建云,孙强,杨玉琴,胡建华,高诚. 东方田鼠肝脏cDNA 文库的构建及部分克隆序列分析[J]. 兽类学报, 2009, 29(4): 447 |
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| 作者姓名： | 冯洁 谢建云 孙强 杨玉琴 胡建华 高诚 |
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| 作者单位： | 上海市实验动物质量监督检验站,上海201203 |
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| 基金项目： | 上海市科学基金资助项目 |
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| 摘 要： | 东方田鼠(Microtus fortis)隶属于啮齿目仓鼠科(Cricetidae)田鼠亚科(Microtinae)田鼠属(Microtus),主要分布于我国西北、东北及南方16个省区.
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| 关 键 词： | cDNA文库 东方田鼠 肝脏 |
Construction and sequence analysis of a cDNA library from the liver of Microtus fortis |
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| FENG Jie,XIE Jianyun,SUN Qiang,YANG Yuqin,HU Jianhua,GAO Cheng. Construction and sequence analysis of a cDNA library from the liver of Microtus fortis[J]. Acta Theriologica Sinica, 2009, 29(4): 447 |
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| Authors: | FENG Jie XIE Jianyun SUN Qiang YANG Yuqin HU Jianhua GAO Cheng |
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| Affiliation: | Shanghai Quality Monitoring Centre for Laboratory Animals,Shanghai 201203,China |
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| Abstract: | The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The ti-ters of this cDNA library were 1076 pfu /ΜL, the recombination rate was about 94% , the library capacity was 1. 08×106. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences, it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice, rats and humans in the Genbank. The cDNA li-brary was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology, fatty liver model development and pathogenesis research of Microtus fortis. |
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| Keywords: | cDNA library Liver Microtus fortis |
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