绿僵菌M189菌株特异性SCAR标记的建立及田间监测应用

为了监测生防真菌绿僵菌菌株在田间释放后的回收，有必要建立菌株DNA分子标记，以此将应用的菌株与其他菌株或田间的土著分离株鉴别开来。作者采用16条随机引物扩增了51株绿僵菌菌株的基因组DNA，得到81个多态性位点。其中M189菌株多态性位点30个，分析得到1个特异性位点，并将该位点的DNA片段测序后转化成为特异性SCAR标记。检验确定了该标记的敏感性，可以从供试的51株菌株中准确鉴定出目的菌株M189。并用该标记检测了从田间回收的3个分离株，确定其中1个与应用菌株M189一致。

Development and application of a specific SCAR marker for detection of a released Metarhizium acridum strain M189 in the field

To monitor the effect of a Metarhizium acridum strain spread in the field, a DNA molecular marker to differentiate a released strain from other strains or native isolates was developed. Sixteen arbitrary primers to amplify the genomic DNA of 51 Metarhizium strains were used and 81 polymorphic sites were obtained. Among them, 30 polymorphic bands were obtained from the strain M189. One band ascertained to be specificity to the strain M189 was sequenced and transformed as a specific SCAR (sequence characterized amplified regions) marker for this strain. The marker sensitivity was tested and confirmed by identifying strain M189 from 51examined strains. The SCAR marker was used to distinguish 3 isolates from the soil samples collected from the field and confirmed one of them being the released strain M189.