Crystallographic structure of the turbine C-ring from spinach chloroplast F-ATP synthase |
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Authors: | Asha?Manikkoth Balakrishna Holger Seelert Sven-Hendric Marx Norbert?A. Dencher Gerhard Grüber |
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Affiliation: | *Nanyang Technological University, School of Biological Sciences, 60 Nanyang Drive, Singapore 637551, Republic of Singapore;†Physikalische Biochemie, Fachbereich Chemie, Technische Universität Darmstadt, Alarich-Weiss-Str.4, D-64287 Darmstadt, Germany |
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Abstract: | In eukaryotic and prokaryotic cells, F-ATP synthases provide energy through the synthesis of ATP. The chloroplast F-ATP synthase (CF1FO-ATP synthase) of plants is integrated into the thylakoid membrane via its FO-domain subunits a, b, b’ and c. Subunit c with a stoichiometry of 14 and subunit a form the gate for H+-pumping, enabling the coupling of electrochemical energy with ATP synthesis in the F1 sector.Here we report the crystallization and structure determination of the c14-ring of subunit c of the CF1FO-ATP synthase from spinach chloroplasts. The crystals belonged to space group C2, with unit-cell parameters a=144.420, b=99.295, c=123.51 Å, and β=104.34° and diffracted to 4.5 Å resolution. Each c-ring contains 14 monomers in the asymmetric unit. The length of the c-ring is 60.32 Å, with an outer ring diameter 52.30 Å and an inner ring width of 40 Å. |
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Keywords: | CF1FO-ATP synthase c-rotor crystallization F-ATP synthase spinach chloroplast |
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