Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis |
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| Affiliation: | 1. Department of Molecular Biology and Genetics, Faculty of Sciences and Arts, Ondokuz Mayis University, 55139 Samsun, Turkey;2. Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, 55139 Samsun, Turkey;3. Department of Biology, Faculty of Science, Eskisehir Technical University, 26555 Eskisehir, Turkey;4. Department of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, Turkey;5. Division of Science Education, Department of Mathematics and Science Education, Gazi University, 06500 Ankara, Turkey;1. Marine College, Shandong University, Weihai, Shandong 264209, PR China;2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, PR China;1. Life Sciences Institute, Zunyi Medical University, Zunyi 563006, PR China;2. Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics (Capital Medical University), Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, PR China;1. Department of Biology, University of Konstanz, Konstanz, Germany;2. Research Training Group R3 – Resilience of Lake Ecosystems, University of Konstanz, Konstanz, Germany;3. Laboratory of Microbiology, Wageningen University & Research, Wageningen, Netherlands;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, Section of Environmental Biotechnology, TU Delft, The Netherlands;3. IAMC-CNR, Spianata S.Raineri 86, 98122 Messina, Italy;4. NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands;5. Faculty of Geosciences, Department of Earth Sciences, Utrecht University, Utrecht, The Netherlands;1. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China;2. China Ocean Sample Repository (Biology), Xiamen 361005, China;3. State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China |
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| Abstract: | Exploration of unexplored habitats for novel actinobacteria with high bioactivity potential holds great promise in the search for novel entities. During the course of isolation of actinobacteria from desert soils, four actinobacteria, designated as 5K548T, 7K502T, 16K309T and 16K404T, were isolated from the Karakum Desert and their bioactivity potential as well as taxonomic provenances were revealed by comprehensive genome analyses. Pairwise sequence analyses of the 16S rRNA genes indicated that the four strains are representatives of putatively novel taxa within the prolific actinobacterial genus Saccharopolyspora. The strains have typical chemotaxonomic characteristics of the genus Saccharopolyspora by having meso-diaminopimelic acid as diagnostic diaminoacid, arabinose, galactose and ribose as whole-cell sugars. Consistent with this assignment, all of the isolates contained phosphatidylcholine in their polar lipid profiles and MK-9(H4) as the predominant menaquinone. The sizes of the genomes of the isolates ranged from 6.0 to 10.2 Mb and the associated G + C contents from 69.6 to 69.7 %. Polyphasic characterizations including determination of overall genome relatedness indices revealed that the strains are representatives of four novel species in the genus Saccharopolyspora. Consequently, isolates 5K548T, 7K502T, 16K404T and 16K309T are proposed as novel Saccharopolyspora species for which the names of Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov. and Saccharopolyspora terrae sp. nov. are proposed, respectively. Comprehensive genome analysis for biosynthetic gene clusters showed that the strains have high potential for novel secondary metabolites. Moreover, the strains harbour many antimicrobial resistance genes providing more evidence for their potentiality for bioactive metabolites. |
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| Keywords: | Actinobacteria Antimicrobial resistance genes Biosynthetic gene clusters Bioactivity Desert |
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