Chemical selection of mutants that affect ADH activity in Drosophila. III. Effects of ethanol |
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Authors: | Charles Vigue William Sofer |
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Institution: | (1) Department of Biology, Johns Hopkins University, Baltimore, Maryland;(2) Present address: Department of Biology, St. Joseph College, West Hartford, Connecticut |
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Abstract: | A chemical selection scheme is presented for the isolation of rare Adh-positive Drosophila. It makes use of the fact that flies lacking detectable ADH activity die as adults or larvae on relatively low concentrations of ethanol in the medium. We have demonstrated that this procedure is a practical one by crossing two Adh-negative alleles, screening 1.5×106 embryos, and isolating 14 Adh-positive survivors.Supported by Postdoctoral Fellowship GM-57 from the National Institutes of Health to C. V. and by Grant GM-18254 from the NIH.Contribution No. 841 from the Department of Biology, The Johns Hopkins University. |
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Keywords: | selective technique alcohol dehydrogenase ethanol Adh-negative mutants |
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