液相色谱紫外-荧光串联检测器法检测我国污水中常见11种抗生素

城市污水处理厂是环境中多种抗生素污染物的主要来源之一.建立污水中多种类抗生素的同时分析检测方法,可为获取该类污染物的常规监管提供技术储备.本研究以我国污水处理厂检出率最高的抗生素为目标污染物,建立了同时分析检测11种抗生素的高效液相色谱紫外/荧光检测器串联分析方法.该方法首先使用紫外检测器,在一组优化仪器参数条件下,实...

Determination of 11 antibiotics frequently detected in municipal wastewater in China by high-performance liquid chromatography with ultraviolet and fluorescence detector

Municipal wastewater plant is an important source of antibiotics in the environment.Development of analytical methods for simultaneous determination of diverse antibiotics in municipal wastewater is crucial for monitoring and regulation of antibiotics.An analytic method based on high-performance liquid chromatography with ultraviolet and fluorescence detector was developed for simultaneously analyzing the presence of 11 antibiotics among four classes of antibiotics most frequently detected in wastewater treatment plants in China.Under a set of optimum analytic parameters,complete separation and detection of the 11 target antibiotics and an internal standard(R>1.5) were obtained by employing an ultraviolet detector.By employing tandem fluorescence detector,lower detection limits were obtained for fluoroquinolone antibiotics.Limits of detection for the 11 target antibiotics ranged from 4.19 to 22.8 μg·L^-1,quantitative limits fell within 5.1-56.4 μg·L^-1,and recovery rates were 43.5%-102.8%.Sewage samples from inlet and outlet of a wastewater treatment plant in Shenyang were analyzed by such method.Sulfadiazine,ciprofloxacin,and norfloxacin were detected in the inlet samples,with concentrations of 440.9,2784.8,and 515.2 ng·L^-1,respectively.In the outlet samples,only ciprofloxacin and norfloxacin were detected,with concentrations of 118.9 and 444.7 ng·L^-1,respectively.This method is convenient,time-saving,low in detection limit,and thus is suitable for regular analysis of antibiotics in wastewater treatment plants in China.