水貂犬瘟热动物模型的建立

目的建立水貂犬瘟热动物模型,并利用水貂犬瘟热模型评价不同犬瘟热强毒株的毒力,为水貂犬瘟热病毒疫苗的研究奠定基础。方法从猴、藏獒、犬的病料中分离犬瘟热病毒,测定犬瘟热病毒的毒力,并进行传代培养。利用犬的犬瘟热动物模型筛选稳定的犬瘟热强毒株,进行水貂犬瘟热动物模型的建立及其毒力评估。结果筛选出了稳定的犬瘟热强毒株并进行了家犬动物实验,同时表现出了强烈的临床症状,并利用不同的代次毒进行了犬瘟热动物模型的建立。结论成功建立了犬瘟热动物模型并对不同来源的犬瘟热病毒毒力进行了评估。

The Mink Model Establishment of Canine Distemper Virus

Objective To establish a mink model of canine distemper virus, and different canine distemper virulent strain virulence was evaluated by the mink animal model for laying the foundation for the study of the canine distemper virus. Methods Different canine distemper viruses were separated from specimens ,cell-cultured, passaged and determined virulence of CDV. And we compared the virulence of different generation virulent strains, stable virulent strains were used for establishing mink canine distemper animal model and evaluated their virulence. Results We screened 3 canine distemper virulent strains and made dog animal experiments, the three viruses infected dogs and they showed a strong clinical symptoms, and mink animal model was established using different generations. Conclusion we successfully established the canine distemper animal model and evaluated virulence of CDV.