改良导管固定法在硬膜外阻滞大鼠模型构建中的应用

目的探讨改良硬膜外导管固定法应用于构建硬膜外阻滞动物模型的可行性及效果。方法采用随机数字法将24只雄性Wistar大鼠分为实验组和对照组,每组12只,用于构建硬膜外阻滞大鼠模型。实验组在切开置管后导管固定使用创新性关卡,采用不透气无菌医用胶带封闭导管;对照组则采用单纯缝线环绕法固定,并保留导管接头固定于后颈部。术后每天硬膜外腔注射0.1%罗哌卡因30 uL,共28 d。对比观察两组术后发生感染率,硬膜外阻滞模型有效时间及硬膜外导管深度变化等情况。结果术后感染发生率：两组间差异无统计学意义（P〉0.05）;模型有效时间：实验组长于对照组（26.2±1.7d＆18.5±3.0d,P〈0.05）;硬膜外腔内导管深度：实验组与对照组比,其均值和离散程度的差异均具有显著性（1.81±0.07＆1.44±0.55,P〈0.05）。结论应用改良导管固定法构建大鼠硬膜外阻滞模型,有助于提高其稳定性和成功率。

Modified Catheter Fixed Method Used to Build Rat Epidural Anesthesia Models

Objective To investigate the feasibility and effect of rat epidural anesthesia models by modifying fixation of the epidural catheter. Methods 24 male Wistar rats, used to build epidural anesthesia models, were randomly divided into experimental group （n = 12 ） and control group （n = 12）. After inserted the catheter, the catheter were fixed by some blocks designed with sterile adhesive tape and the catheter connector were replaced by sterile medical tape in the experimental group, while catheter was fixed with wire wound and catheter connector was sutured on the back of the neck in the control group. O. 1% ropivacaine 30ul was injected into epidural space once a day and continued 28 days. Then incidence of postoperative infection, the time to failure of the epidural anesthesia models was recorded and the epidural catheter depth measured on the 28th day. Results No significant difference was noted in the incidence of postoperative infection between two groups （P 〉 0.05）. The effective duration of models in the experimental group is significantly longer than that in the control group（26.2 ＋ 1.7d ＆ 18.5 ~ 3.0d,P 〈 0. 05 ）. The epidural catheter depth were 1.81 ＋ 0. 07cm in the experimental group, respectively, whereas in the control group, 1.44 ＋ 0. 55cm of the depth were detected, the degree of dispersion is lower than that in the control group （ P 〈 0.05 ）. Conclusion Modified catheter fixation can significantly improve the stability and success rate of epidural anesthesia animal models.