胸4～5椎间隙直视下硬膜外头端置管建立上胸段硬膜外阻滞大鼠模型

目的为上胸段硬膜外阻滞的动物试验研究提供经济、稳定、可靠的模型。方法以大鼠为研究对象,经胸4～5椎间隙,直视下向头端于硬膜外腔置入经拉撕等处理后的临床用硬膜外导管,采用椎旁肌肉缝扎导管,皮下隧道预留缓冲长度等方法固定导管。48 h后大鼠硬膜外腔注入美蓝100μL/kg,尸检鉴定模型成功和药液的分布范围。结果置管48 h后的成功率为85.8%,硬膜外腔的感染率为0,导管脱出几率5%。结论本研究证实了经胸4～5椎间隙直视下头端置管建立上胸段硬膜外阻滞大鼠模型的可行性,并具有损伤小、稳定性好、成功率高、费用低及周期短等优点。

Establishment of an Upper Thoracic Epidural Anesthesia Model in Rats through Th4-Th5 Cephalad Catheterization

Objective To establish an upper thoracic epidural anesthesia model in rats.Methods A total of 120 rats were used in this study.Rats were fixed in a prone position,and an epidural catheter was implanted into the spinal epidural space through the T4-5 joint in cephalad direction.At 48 hours after catheterization,100 μL/kg methylene blue was injected into the epidural cavity in rats to evaluate whether the catheterization was successful or not,and to identify the location and distribution of the drug solution.Results The thoracic epidural anesthesia model was successfully performed in 85.8% of the rats.The infection rate of the epidural cavity was 0,and the rate of catheter prolapse was 5% after 48 hours.Conclusions T4-5 cephalad catheterization is feasible to provide a stable upper thoracic epidural anesthesia model in rats,with apparent advantages such as mild traumatic injury,good stability,high success rate,lower cost,and less time consuming.