An improved micro-method for avidin assay (Short Communication) |
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| Authors: | Heikki Elo and Pentti Tuohimaa |
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| Institution: | Department of Anatomy, University of Turku, 20520 Turku 52, Finland |
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| Abstract: | A sensitive method for avidin assay was devised. Tritiated biotin is bound to avidin and this complex is then bound to bentonite. Radioactivity is converted into a gas form by combustion and counted in an automatic proportional counter with 55% efficiency and background of 3.7c.p.m. The sensitivity is 1-2ng of avidin. |
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