Effects of GABA on acutely isolated neurons from the gustatory zone of the rat nucleus of the solitary tract |
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Authors: | Du, J Bradley, RM |
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Affiliation: | Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109-1078, USA. |
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Abstract: | Responses of acutely isolated neurons from the rostral nucleus of thesolitary tract (rNST) to GABA receptor agonists and antagonists wereinvestigated using whole-cell recording in current clamp mode. The isolatedneurons retain their morphology and can be divided into multipolar,elongate and ovoid cell types. Most rNST neurons (97%), including all threecell types, respond to GABA with membrane hyperpolarization and a reductionin input resistance. The GABA(A) receptor agonist muscimol reduces neuronalinput resistance in a concentration-dependent manner, whereas the GABA(B)receptor agonist baclofen had no effect on any of the neurons tested. TheGABA and muscimol reversal potentials were both found to be -75 mV Both theGABA competitive antagonist picrotoxin and the GABA(A) receptor antagonistbicuculline block the effect of GABA in a concentration-dependent manner.These results suggest that GABA activates all neurons in the rNST and thatinhibitory synaptic activity is important in brainstem processing ofgustatory and somatosensory information. |
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