Interleukin-1beta up-regulates expression of neurofilament light in human neuronal cells |
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Authors: | Rempel H Kusdra L Pulliam L |
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Affiliation: | Department of Laboratory Medicine, Veterans Affairs Medical Center, San Francisco, California 94121, USA. |
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Abstract: | Elevated expression of interleukin-1 (IL-1beta), a pro-inflammatory cytokine secreted by activated microglia, is a pathogenic marker of numerous neurodegenerative processes including Alzheimer's disease (AD). We have characterized a link between IL-1beta and the 68-kDa neurofilament light (NF-L) protein, which is a major component of the neuronal cytoskeleton. Using human brain aggregate cultures, we found that IL-1beta treatment significantly increased NF-L expression in primary neurons. Analysis of mRNA levels demonstrated elevated NF-L expression within 72 h while imaging of neurons by immunofluorescent staining for NF-L confirmed IL-1beta-induced NF-L protein expression. These observations suggest a potential inflammatory-induced mechanism for deregulation of an important cytoskeletal protein, NF-L, possibly leading to neuronal dysfunction. |
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Keywords: | gene expression human brain interleukin-1 neurofilament neuro-inflammation |
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