| 长半衰期重组人超氧化物歧化酶的研制及性质 |
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| 引用本文： | 骆训懿,王晶翼,谢邦铁,李战青,刘晓琳,陈晓穗.长半衰期重组人超氧化物歧化酶的研制及性质[J].生物化学与生物物理进展,1994,21(3):234-237. |
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| 作者姓名： | 骆训懿 王晶翼 谢邦铁 李战青 刘晓琳 陈晓穗 |
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| 作者单位： | 海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室;海军总医院分子生物学研究室 |
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| 摘 要： | 一种双亲有机化合物聚苯乙烯马来酸丁酯(SMA)经酰胺键与重组人铜锌超氧化物歧化酶(rhCu/Zn SOD)共价交联,制得修饰酶.当42%游离氨基被修饰时,保留酶活力为88%.酶蛋白主链结构在修饰前后变化不大.与天然酶相比,修饰酶的生物半衰期延长了22倍,抗蛋白水解酶能力亦有所增强.
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| 关 键 词： | 超氧化物歧化酶 蛋白质化学修饰 聚苯乙烯马来酸丁酯 基因工程 |
| 收稿时间： | 1993/3/31 0:00:00 |
| 修稿时间： | 1993/8/16 0:00:00 |
Preparation and Properties of Long Half-Life Recombinant Human Superoxide Dismutase |
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| Luo Xunyi,Wang Jingyi,Xie Bangtie,Li Zhanqing,Liu Xiaolin and Chen Xiaosui.Preparation and Properties of Long Half-Life Recombinant Human Superoxide Dismutase[J].Progress In Biochemistry and Biophysics,1994,21(3):234-237. |
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| Authors: | Luo Xunyi Wang Jingyi Xie Bangtie Li Zhanqing Liu Xiaolin and Chen Xiaosui |
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| Institution: | Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital;Laboratory of Molecular Biology, Naval General Hospital |
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| Abstract: | Recombinant human Cu/Zn SOD(rhCu/Zn SOD) obtained from E.coli was covalently linked with an amphipathic molecule poly-(styrene-co-maleic acid) butyl ester (SMA) via amide linkage. When 42% free amino groups of the enzyme were modified. 88% remained enzyme activity was obtained.The results of circular dichroism of rhCu/Zn SOD and SMA-rhCu/Zn SOD indicated that the structure of the modified rhCu/Zn SOD was scarcely changed. Its biological half-life in blood was prolonged 22 times.and its abilities to resist pepsin and trypsin were increased significantly. |
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| Keywords: | rhCu/Zn SOD chemical modification of protein SMA genetic engineering |
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