Transient suppression of F-plasmid incompatibility in a strain of Escherichia coli |
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Authors: | Radhika Warrier and R. Jayaraman |
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Affiliation: | (1) School of Biological Sciences, Madurai Kamaraj University, 625 021 Madurai, India;(2) Present address: Department of Biology, Carleton University, Ottawa, Canada |
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Abstract: | Summary The incompatibility between F plasmids is transiently suppressed in Escherichia coli strain CSH54. As a result this strain is able to maintain two F factors or an F factor and a mini-F plasmid for considerably longer periods than normal strains. When selective pressure for two markers carried by two separate Fs (or an F and mini-F) is imposed on normal strains, the two plasmids tend to form a cointegrate structure which can be detected genetically by the joint transfer of both the markers upon mating. This does not happen in CSH54; instead, the two plasmids are maintained and transferred independently. Physical evidence for the maintenance of an F and a mini-F plasmid is provided by agarose gel electrophoresis. |
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Keywords: | F plasmid Incompatibility |
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