Transforming activity of plasmid and chromosomal DNA inEscherichia coli |
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Authors: | M ?rogl J Hochmannová J Ne?vera J ?tokrová M Klégr |
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Institution: | (1) Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4;(2) Institute of Molecular Genetics, 166 10 Prague 6 |
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Abstract: | An auxotrophic strain ofEscherichia coli with therecB recC sbcB genotype was transformed by chromosomal DNA of the prototrophic strain and by plasmid DNA carrying genes for antibiotic resistance
(R1drd 19). The donor plasmid DNA obtained by cell lysis in the presence of Triton X-100 and subsequent centrifugation in a caesium
chloride-ethidium bromide gradient was shown to have a circulaf molecule and to retain its completeness after penetration
into the recipient. Experiments with mixtures or plasmid and chromosomal DNA indicate a competition between these two DNA
types during the transformation reaction in the given system. |
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