Mapping B-cell epitopes of hepatitis C virus E2 glycoprotein using human monoclonal antibodies from phage display libraries |
| |
Authors: | Bugli F Mancini N Kang C Y Di Campli C Grieco A Manzin A Gabrielli A Gasbarrini A Fadda G Varaldo P E Clementi M Burioni R |
| |
Institution: | Istituti di Microbiologia, Facoltà di Medicina e Chirurgia, Università Cattolica del S. Cuore, 00168 Rome, Italy. |
| |
Abstract: | Clinical and experimental evidence indicates that the hepatitis C virus (HCV) E2 glycoprotein (HCV/E2) is the most promising candidate for the development of an effective anti-HCV vaccine. Identification of the human epitopes that are conserved among isolates and are able to elicit protective antibodies would constitute a significant step forward. This work describes the mapping of the B-cell epitopes present on the surface of HCV/E2, as recognized by the immune system during infection, by the analysis of the reciprocal interactions of a panel of human recombinant Fabs derived from an HCV-infected patient. Three unrelated epitopes recognized by antibodies with no neutralization-of-binding (NOB) activity were identified; a fourth, major epitope was defined as a clustering of minor epitopes recognized by Fabs endowed with strong NOB activity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|