Introduction of hygromycin resistance inLotus spp. throughAgrobacterium rhizogenes transformation |
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Authors: | Francesco Damiani Elena Nenz Francesco Paolocci Sergio Arcioni |
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Affiliation: | (1) Istituto Miglioramento Genetico delle Piante Foraggere CNR, Via Madonna Alta, I30 06128 Perugia, Italy |
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Abstract: | The speciesLotus corniculatus andL. tenuis were transformed with anAgrobacterium rhizogenes binary vector, conferring resistance to the antibiotic hygromycin. Transgenic plants recovered from both species were tested for the ability of leaf-derived calluses to grow in a hygromycin-supplemented medium. Molecular analysis showed the integration of the Ri T-DNA and of the gene for hygromycin resistance, with a high frequency of co-transformation. Progeny analysis of the hygromycin resistance indicated this to be a single Mendelian trait in test plants. The transformed plants will be utilized in somatic hybridization experiments with lucerne for producing non-bloating genotypes with condensed tannins in leaves. |
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Keywords: | Lotus Agrobacterium rhizogenes transformation hygromycin resistance tannins |
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