Evaluation of Lespedeza germplasm genetic diversity and its phylogenetic relationship with the genus Kummerowia |
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| Authors: | M L Wang J A Mosjidis J B Morris Z B Chen N A Barkley G A Pederson |
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| Institution: | (1) Plant Genetic Resources Conservation Unit, USDA-ARS, 1109 Experiment Street, Griffin, GA 30223, USA;(2) Department of Agronomy and Soils, Auburn University, 202 Funchess Hall, Auburn, AL 36849, USA;(3) Department of Crop and Soil Sciences, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA |
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| Abstract: | The genetic diversity of the genus Lespedeza is not well known and the phylogenetic relationship of Lespedeza with the genus Kummerowia is unclear. We report the first study in which polymorphic expressed sequence tag-simple sequence repeat (EST-SSR) markers
derived from Medicago, cowpea and soybean were used to assess the genetic diversity of the USDA Lespedeza germplasm collection and clarify its phylogenetic relationship with the genus Kummerowia. Phylogenetic analysis partitioned 44 Lespedeza accessions into three main groups some of which were species-specific and eight subgroups. This data set revealed some misidentified
accessions, and indicated that the two species in the genus Kummerowia are closely related to the genus Lespedeza. Morphological reexamination was used to correct the misidentified accessions within the genus Lespedeza. Our results demonstrated that phylogenetic analysis with morphological reexamination provides a more complete approach to
classify accessions in plant germplasm collection and conservation. |
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| Keywords: | Lespedeza Kummerowia Germplasm SSR Genetic diversity Phylogeny |
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