Genetic diversity and population differentiation of chestnut blight fungus, Cryphonectria parasitica, in China as revealed by RAPD |
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Authors: | Boqian Yan Zuozhou Li Hongwen Huang Ling Qin |
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Institution: | (1) Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuhan, Hubei, 430074, P.R. China;(2) Beijing University of Agriculture, Beijing, 102206, P.R. China |
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Abstract: | Seventeen Cryphonectria parasitica populations sampled from six regions in China were investigated using RAPD. Across all 169 isolates from the 17 populations
evaluated, 52 of the 71 markers (73%) were polymorphic, total genetic diversity (h) was 0.1463, and Shannon’s index was 0.2312. Diversity within populations accounted for 74% of total genetic diversity, and
genetic differentiation among populations was 0.26 (G
ST = 0.26). Gene flow was 1.4 among the populations; higher gene flow was found among populations within regions and among regions
N
m (G
SR) = 2.8 and N
m (G
RT) = 3.5]. The unweighted pair group mean analysis (UPGMA) dendrogram revealed two distinct clusters: the northern China group
and the southern China group. The spatial autocorrelation analysis revealed that the variation at most loci was randomly distributed
and lacked spatial structure, but several loci and closer distances were spatially structured. Human activity and habitat
could also be important factors affecting genetic structure among C. parasitica populations in China. Genetic diversity was highest in Southwest China, descending in an orderly fashion to Northeast China.
This pattern indicated that Southwest China might be the center of origin of C. parasitica in China. The present study provides useful information for understanding the origin and spread of chestnut blight fungus
in China and valuable data for formulating relevant strategies for controlling the disease in China. |
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Keywords: | Cryphonectria parasitica Genetic diversity Population genetic structure Gene flow Spatial autocorrelation |
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