Tetrapeptides on N- and C-Terminal Regions of Mastoparan Inhibit Catecholamine Release from Chromaffin Cells by Blocking Nicotinic Acetylcholine Receptor |
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Authors: | Noriyuki Monoi Masaya Usui Shunichi Takahashi Hisashi Ito Konosuke Kumakura Hisataka Kasai |
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Institution: | Graduate School of Science and Engineering, Aoyama Gakuin University, Fuchinobe, Sagamihara, Kanagawa, Japan. monoi1@lion.co.jp |
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Abstract: | Mastoparan (MP), a tetradecapeptide in wasp venom, has been reported to evoke catecholamine release, but also reported to inhibit secretory response upon nicotinic stimulation in adrenal chromaffin cells. To elucidate the inhibitory mechanism of MP, we examined the effect of two MP fragments (INLK-NH2 and KKIL-NH2) on catecholamine release in bovine adrenal chromaffin cells. These MP fragments inhibited catecholamine release induced by nicotinic stimulation in a noncompetitive manner. These fragments did not affect catecholamine release evoked by high K+] or by other secretagogues, neither caused catecholamine release by themselves. Replacement by hydrophobic and basic amino acids of the MP fragments enhanced the inhibitory effects on ACh-evoked catecholamine release. Among 23 analogs of the MP fragments, (Nle)3-R-NH2 showed the most potent inhibition with IC50 = 541 microM. These results suggested that the MP fragments selectively inhibit the secretory response to nicotinic stimulation by attacking nAChR on the site(s) made up of hydrophobic and acidic amino acids but other than ACh-binding sites. This mechanism may explain the inhibitory action of MP on nicotine-evoked catecholamine release. |
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Keywords: | mastoparan fragments nicotinic acetylcholine receptor chromaffin cells catecholamine mastoparan-fragment analogs mastoparan |
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