Unilamellar vesicles as potential capreomycin sulfate carriers: Preparation and physicochemical characterization |
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Authors: | Stefano Giovagnoli Paolo Blasi Claudia Vescovi Giuseppe Fardella Ione Chiappini Luana Perioli Maurizio Ricci Carlo Rossi |
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Institution: | (1) Department of Chemistry and Technology of Drugs, Università degli Studi di Perugia, Via del Liceo 1, 06123 Perugia, Italy |
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Abstract: | The aim of this work was to evaluate unilamellar liposomes as new potential capreomycin sulfate (CS) delivery systems for
future pulmonary targeting by aerosol administration. Dipalmitoylphosphatidylcholine, hydrogenated phosphatidylcholine, and
distearoylphosphatidylcholine were used for liposome preparation. Peptide-membrane interaction was investigated by differential
scanning calorimetry (DSC) and attenuated total internal reflection Fourier-transform infrared spectroscopy (ATIR-FTIR). Peptide
entrapment, size, and morphology were evaluated by UV spectrophotometry, photocorrelation spectroscopy, and transmission electron
microscopy, respectively. Interaction between CS and the outer region of the bilayer was revealed by DSC and ATIR-FTIR. DSPC
liposomes showed enhanced interdigitation when the CS molar fraction was increased. Formation of a second phase on the bilayer
surface was observed. From kinetic and permeability studies, CS loaded DSPC liposomes resulted more stable if compared to
DPPC and HPC over the period of time investigated. The amount of entrapped peptide oscillated between 10% and 13%. Vesicles
showed a narrow size distribution, from 138 to 166 nm, and a good morphology. These systems, in particular DSPC liposomes,
could represent promising carriers for this peptide. |
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Keywords: | capreomycin sulfate liposomes DSC ATIR-FTIR phase transition |
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