Identification of a novel site specific endonuclease produced by Mycoplasma fermentans: discovery while characterizing DNA binding proteins in T lymphocyte cell lines. |
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| Authors: | N F Halden J B Wolf W J Leonard |
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| Affiliation: | Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892. |
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| Abstract: | We have discovered a new restriction endonuclease, MfeI, in nuclear extracts from T cells contaminated with Mycoplasma fermentans. This endonuclease was identified while studying proteins binding to the interleukin-2 receptor alpha chain gene promoter. MfeI cuts at the recognition sequence C'AATTG generating EcoRI compatible cohesive ends. Potential applications are discussed. |
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