Production of inulo-oligosaccharides using endo-inulinase from a pseudomonas sp. |
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Authors: | Dong Hyun Kim Yong Jin Choi Seung Koo Song Jong Won Yun |
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Affiliation: | (1) Dept. of Biotechnology, Taegu University, Kyungbuk, 712–714, Korea;(2) Dept. of Genetic Engineering, Korea University, Seoul, 132, Korea;(3) Dept. of Chemical Engineering., Pusan National University, Pusan, 609–735, Korea |
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Abstract: | Inulo-oligosaccharides were produced from inulin by using high activities of an endo-acting inulinase. The total yields of oligosaccharide were slightly decreased as the concentration of inulin increased from 50 to 200g/l. Under the optimal reaction conditions, the products consist of inulo-oligosaccharides ranging from DP (degrees of polymerization)2 to DP7, where the major oligosaccharides are 29.8% DP2, 21.4% DP3, and 8.1% DP4 oligomer, respectively. The maximum yield was 75.6% when 50g inulin/l and 15 units/g substrate were used. |
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