Attenuation of LDL receptor gene expression by selenium deficiency during hypercholesterolemia |
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Authors: | Sanjiv Dhingra Mohinder P Bansal |
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Institution: | (1) Department of Biophysics, Panjab University, Chandigarh, 160014, India |
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Abstract: | Selenium deficiency has been associated with hypercholesterolemia. Present study was aimed to determine the effect of selenium
(Se) deficiency on LDL receptor (LDL-R) activity as well as mRNA expression during experimental hypercholesterolemia in SD
male rats. Animals were fed Se adequate (0.2 ppm) and deficient (0.02 ppm) control diet as well as high cholesterol (2%) diet
(HCD) for 1 and 2 months. LDL-R activity was measured in vivo by injecting radiolabeled LDL to rats and percent decrease in cpm with time was taken as a measure of LDL clearance and in
turn LDL-R activity. LDL-R mRNA expression was studied by RT-PCR. LDL-R activity and mRNA expression decreased significantly
on HCD feeding in both Se deficient and adequate diet fed rats after 2 months. In Se deficiency receptor activity and mRNA
expression decreased significantly. After 2 months LDL-R activity and expression decreased in both the Se deficient groups
and in Se adequate HCD fed group in comparison to 1 month data. But after 4 month there was no significant difference observed
in LDL-R activity and mRNA expression in selenium deficiency as well as on HCD feeding. So the present results demonstrate
that Se deficiency act synergistically with hypercholesterolemia to downregulate LDL-R activity as well as mRNA expression. |
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Keywords: | cholesterol hypercholesterolemia LDL-R selenium |
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