Formation of [3H, 32P]phytic acid in germinating wheat |
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| Authors: | E Graf |
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| Affiliation: | Northern Regional Research Center, Agricultural Research Service, U. S. Department of Agriculture, Peoria, Illinois 61604 USA |
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| Abstract: | Doubly labeled phytic acid of high specific activity was prepared by incubating whole wheat seeds with [32P]phosphoric acid and [3H]myoinositol for 48 h and purifying by anion-exchange chromatography on AG 1- X 8 resin. Both degradation and synthesis of phytic acid were inhibited by KF to a similar extent, yet the catabolic and anabolic pathways involved distinctly different enzyme systems, as no [3H, 32P]myoinositol tetra- or pentaphosphate could be detected. |
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