Evidence for a constitutive cytoplasmic cutinase in ungerminated conidia of Botrytis cinerea Pers.: Fr. |
| |
Authors: | Katia Gindro Roger Pezet |
| |
Affiliation: | Swiss Federal Agricultural Research Station of Changins, Route de Duillier, CH-1260 Nyon, Switzerland |
| |
Abstract: | Cytoplasmic soluble proteins from ungerminated conidia of Botrytis cinerea exhibited cutinase activity, while cell wall binding proteins lacked this activity. Cutinase activity in proteins extracted from cell walls and cytoplasm of ungerminated conidia of Botrytis cinerea was determined using p-nitrophenyl butyrate (PNB) and TLC analysis of products derived from hydrolysis of [3H]cutin. Treatment of conidia with indoxyl acetate, a substrate indicative of non-specific esterase and cutinase activity, also gave a positive reaction in the cytoplasm of ungerminated conidia. The possible role of a putative constitutive cutinase in the cytoplasm of conidia in the early stages of infection of plants by B. cinerea is discussed. |
| |
Keywords: | Botrytis cinerea Esterase Cutinase [3H]Cutin |
本文献已被 ScienceDirect 等数据库收录! |
|