Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells |
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| Authors: | Carvalho Pedro P Wu Xiying Yu Gang Dietrich Marilyn Dias Isabel R Gomes Manuela E Reis Rui L Gimble Jeffrey M |
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| Affiliation: | Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808, USA. |
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| Abstract: | Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable. |
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