放线菌L74溶藻物质的分离及其溶藻特性

为了得到溶藻物质并进行溶藻特性的研究, 采用离心、乙醇沉淀和有机溶剂萃取的方法从发酵液中粗提得到溶藻物质, 再通过硅胶吸附柱层析和透析进行精制, 并采用试管试验法对其进行分析。此外研究了溶藻物质的pH稳定性、热稳定性以及对藻类抗氧化酶系统的影响。该菌株的溶藻物质具有较大极性, 且分子量小于3 kD; 经化学法鉴定, 基本确定为糖苷类物质、内酯类物质和萜类物质中的一种或几种的组合; 具酸碱稳定性, 但对热敏感, 随着处理温度的升高, 溶藻效果逐渐下降, 当温度超过90 °C处理1 h后, 活性几乎全部丧失;

Isolation and algicidal characteristics of the algicidal components from actinomycete strain L74

To obtain the algicidal components from actinomycete strain L74, a series of techniques including centrifugation, ethanol precipitation, organic solvents extraction, silica gel adsorption chromatography, dialysis and chemical analysis were used. In addition, we studied the characteristic of stabilities of algicidal components under different pH and temperatures, and the influence of it to anti-oxidation enzyme system of Microcstis aeruginosa. The results suggested that the algicidal components may be or contain glycosides, lactones and triterpenes, which the molecular weights are less than 3.0 kD. In extraction experiments, Petroleum ether, acetic ether and n-Butanol can efficiently separate the algicidal components into water-layer. The algicidal components are stable for pH, but sensitive for temperature. After treated under 90 °C for one hour, the activity is nearly lost. When cultivated with algicidal components, the MDA contents of Microcstis aeruginosa increase and maintain at a high level, but the activities of SOD, POD and CAT significantly increase in the early cultivation and decline quickly in the late stage.