A comparative study of different cytokinins on the formation of Rhododendron forrestii Balf. f. ex diels. axillary shoots in vitro |
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| Authors: | Aurelia Kamenicka Jozef Valka Gabriela Vizarova |
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| Institution: | (1) Institute of Forest Ecology, Slovak Academy of Sciences, Sturova 2, 960 53 Zvolen, Slovak Republic;(2) Institute of Microbiology, Slovak Academy of Sciences, Stefanikova 3, 814 34 Bratislava, Slovak Republic |
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| Abstract: | The effect of different concentrations and activities of cytokinins on the morphogenesis of regenerated Rhododendron forrestii Balf. f. ex Diels. shoots taken from nodal segments were tested. We evaluated zeatin, zeatin riboside, izopentyladenine,
izopentyladenine riboside, kinetin, kinetin riboside, benzylaminopurine, benzylaminopurine riboside. The experimental results
were evaluated by mathematical methods and regression analysis describing the effect of isoprenic and aromatic type of cytokinins.
On the basis of this modelling, maximum axillary shoot production was attained with medium supplemented with 2.0 mg·l−1 izopentyladenine riboside, 2.0 mg·l−1 benzylaminopurine and 20 g·l−1 sucrose. Minimal axillary shoots were produced with kinetin and kinetin riboside. |
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| Keywords: | cytokinins axillary shoots Rhododendron forrestii shoot proliferation |
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