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Quantitative electron probe X-ray microanalysis of thin objects using an independent mass determination method
Affiliation:1. Key Laboratory of Knowledge Automation for Industrial Processes of Ministry of Education, School of Automation and Electrical Engineering, University of Science and Technology Beijing, Beijing, 100083, China;2. Institute of Artificial Intelligence, University of Science and Technology Beijing, Beijing, 100083, China
Abstract:Elemental mass fraction determinations on thin biological specimens by quantitative electron probe X-ray microanalysis commonly use a total mass estimation based on registration of X-ray continuum. We have tested whether the accurary of such determinations on individual biological objects can be improved when a densitometric method for mass determination is used. Air-dried femtoliter droplets with dry masses in the order of 10−16 kg were used as model objects. An improvement of over two-fold upon the continuum method could be achieved.
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