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DMSO-induced terminal differentiation and trisomy 15 in myeloid cell line transformed by the Rauscher murine leukemia virus
Affiliation:1. Section of Stem Cell Biology, Division of Oncology, Department of Medicine, Washington University in St. Louis School of Medicine, St. Louis, MO;2. Developmental, Regenerative and Stem Cell Biology Program, Division of Biology and Biomedical Sciences, Washington University in St. Louis School of Medicine, St. Louis, MO;1. Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, VIB, Herestraat 49, 3000 Leuven, Belgium;2. Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, KU Leuven and Leuven Cancer Institute (LKI), Herestraat 49, 3000 Leuven, Belgium;1. Biozentrum, University of Basel, Basel, Switzerland;2. Department of Biomedicine, University and University Hospital of Basel, Basel, Switzerland;3. Division of Infectious Diseases, University and University Hospital of Basel, Switzerland;4. Swiss Institute of Bioinformatics, sciCORE Computing Center, University of Basel, Basel, Switzerland;5. Novartis, Basel, Switzerland;6. Department of Paediatrics, University of Oxford, Oxford, UK;7. Helmholtz Center for Infection Research, Braunschweig, Germany;8. Division of Infectious Diseases and Department of Medicine, Cantonal Hospital of Winterthur, Winterthur, Switzerland;9. Université Paris Descartes–Sorbonne Paris Cité, Institut Imagine, Paris, France and APHP Hôpital Universitaire Necker-Enfants Malades, Unité d’Immunologie-Hématologie et Rhumatologie Pédiatrique, Paris, France;1. Division of Clinical Pharmacology, Toxicology and Therapeutic Innovation, Children''s Mercy Kansas City, University of Missouri–Kansas City School of Medicine, Kansas City, Missouri;2. Medical College of Wisconsin, Milwaukee, Wisconsin;3. RPRD (Right Patient Right Drug) Diagnostics, LLC, Wauwatosa, Wisconsin;4. Agena Bioscience, San Diego, California;5. Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York;6. Sema4, Stamford, Connecticut;7. Department of Pathology, University of Utah, Salt Lake City, Utah;11. ARUP Laboratories, Salt Lake City, Utah;12. Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana;8. Informatics and Data Science Branch, Division of Laboratory Systems, Office of Surveillance, Epidemiology, and Laboratory Services, Centers for Disease Control and Prevention, Atlanta, Georgia
Abstract:A new myeloid cell line was isolated from a myeloid leukemia obtained after infection of BALB/c mice with Rauscher murine leukemia virus (R-MuLV). After syngeneic transplantation of leukemic cells tumor formation was induced. Of one of these tumors a permanent cell line could be established. The cells grow in suspension culture with a doubling time of 18 h and morphologically and cytochemically show all the characteristics of myelocytes. The cells carry trisomy of chromosome 15. These cells prove to be completely independent of colony stimulating activity (CSA) regarding both their growth and their differentiation capacity.One of the main characteristics of this cell line is its inducibility for terminal differentiation after treatment with dimethylsulfoxide varying in concentrations from 0.5% to 1.5%. After two days metamyelocytes and after three to four days granulocytes and macrophages formed. The differentiation of these cells goes together with an increase of lysosomal enzyme activities like β-N-acetylglucosaminidase and lysozyme.
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