Growth of adult human cells in culture at clonal densities |
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| Affiliation: | 1. Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;2. Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;3. Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;4. Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL 62901, USA;1. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada;2. Departments of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada;3. Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada;4. Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada;1. College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, 77843-4466, United States;2. Laboratory of Animal Reproduction, Faculty of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, MG, 38400-902, Brazil;1. ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Switzerland;2. Animal Nutrition, Institute of Agricultural Sciences, Switzerland;1. Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;2. Department of Veterinary Science, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil |
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| Abstract: | The effect of varying medium concentrations of fetal calf serum (FCS), horse serum (HS) and chicken embryo extract (EE) on the growth capacity of adult human cells cultured at clonal densities was investigated. The best growth was consistently obtained with medium containing 20 parts FCS and 2 parts heat-treated commercial EE. The growth potential of human muscle cells was not related to the age of the patient from which the biopsy was taken, but was dependent on the microenvironment of the culture and the cell density. |
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