Autofluorescence in freshly isolated adult human liver sinusoidal cells |
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| Authors: | Anett Kristin Larsen Jaione Simn-Santamaría Kjetil Elvevold Bo Gran Ericzon Kim Erlend Mortensen Peter McCourt Brd Smedsrd Karen Kristine Srensen |
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| Institution: | 1.Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway;2.Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska University Hospital, Stockholm, Sweden;3.University Hospital of North Norway, Tromsø, Norway |
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| Abstract: | Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of exogenous fluorophores, especially when labelling of live cells where the quencher is not compatible.Key words: Autofluorescence, endogenous fluorophores, liver, endothelial cells, lipofuscin |
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